研究目的
To establish a rapid, reliable, low-cost, and highly sensitive method to measure intracellular and extracellular H2O2 levels for studying the biological effects of H2O2, particularly using an electrochemiluminescent biochip integrated with AuNPs-loaded mesoporous silica film to avoid aggregation issues in live cell environments.
研究成果
The AuNPs-loaded MSF biochip is a low-cost, reproducible, and sensitive tool for H2O2 detection with a linear range of 0.1–200 μM and LOD of 25.3 nM. It successfully detected H2O2 from macrophage cells and assessed antioxidant activity, showing promise for biosensing and drug development applications.
研究不足
The paper does not explicitly mention specific limitations, but potential areas for optimization could include long-term stability in varied biological environments, scalability for mass production, and interference from other cellular components not tested.
1：Experimental Design and Method Selection:
The study designed a miniaturized ECL biochip using AuNPs-loaded mesoporous silica film (MSF) on ITO glass to detect H2O
2：The MSF serves as a template to prevent AuNPs aggregation, and ECL is used for high-throughput analysis with a CCD camera. Sample Selection and Data Sources:
Chemicals were purchased from suppliers like Sigma-Aldrich and Alfa Aesar; RAW
3：7 macrophage cells were used as a model for H2O2 release studies. List of Experimental Equipment and Materials:
2 Equipment includes CHI 850D electrochemical station, BPCL chemiluminescence analyzer, Tanon 5200 imaging system, JEM-200CX TEM; materials include TEOS, CTAB, HAuCl4, luminol, ITO glass, etc.
4：Experimental Procedures and Operational Workflow:
MSF was synthesized on ITO using St?ber-solution growth, functionalized with TMAC, loaded with AuNPs via reduction with NaBH
5：The biochip was fabricated with patterned ITO and adhesive tape. ECL measurements were performed by adding samples to reservoirs and applying voltage. Data Analysis Methods:
ECL images were analyzed using Image J software to calculate gray values; CV and ECL intensity were measured for characterization.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
