研究目的
To develop a novel multifunctional nanoplatform for combined chemo-photothermal cancer therapy to enhance therapeutic efficacy and reduce side effects.
研究成果
The ACA nanocomplex effectively combines chemo-photothermal therapy, significantly inhibiting tumor growth up to 95%, prolonging survival, and eradicating microscopic residual tumors as shown by PET imaging. It reduces side effects associated with separate therapies and offers a promising strategy for synergistic cancer treatment.
研究不足
The study is limited to CT26 colorectal tumor model in mice; applicability to other cancer types or human models is not established. Potential limitations include the use of intraperitoneal injection which may not fully mimic clinical routes, and the specific laser parameters (532 nm) might not be optimal for all scenarios. Optimization of dosing and timing could be further investigated.
1：Experimental Design and Method Selection:
The study designed a thermo-responsive alginate nanogel co-loaded with gold nanoparticles and cisplatin (ACA nanocomplex) for combined therapy. Methods included synthesis, characterization, in vivo biodistribution, antitumor efficacy assessment, thermometry, and PET imaging.
2：Sample Selection and Data Sources:
CT26 colorectal cancer cells and BALB/c mice were used. Cells were cultured in RPMI 1640 medium, and tumors were induced subcutaneously.
3：List of Experimental Equipment and Materials:
Materials included hydrogen tetrachloroaurate(III) trihydrate, alginic acid sodium salt, cisplatin, fetal bovine serum, RPMI 1640 medium, etc. Equipment included TEM (LEO906-ZEISS), DLS (Malvern Zetasizer Nano ZS-90), UV-visible spectrometer (Rayleigh UV-1601), ICP-MS (ELAN DRC-e spectrometer), laser system (532 nm, Changchun New Industries Optoelectronics Tech), IR thermal camera (Testo 875–1i), micro-PET scanner (Xtrim PET).
4：Experimental Procedures and Operational Workflow:
Synthesis of ACA nanocomplex, characterization via TEM, DLS, zeta potential, UV-vis. Tumor induction in mice, biodistribution study via ICP-MS at 6, 12, 24 h post-injection. Antitumor study with six treatment groups, laser irradiation at 532 nm, 1.1 W/cm2 for 15 min. Thermometry with IR camera, thermal dose calculation. PET imaging with [18F]FDG.
5：1 W/cm2 for 15 min. Thermometry with IR camera, thermal dose calculation. PET imaging with [18F]FDG.
Data Analysis Methods:
5. Data Analysis Methods: Tumor volume calculation, thermal dose calculation using CEM 43°C formula, statistical analysis of tumor growth and survival rates, PET parameter extraction (SUV, MTV, TLG).
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