研究目的
To design and synthesize a probe (probe 2) for the selective and sensitive fluorescence "turn-on" detection of Al3+ ions by combining aggregation-induced emission enhancement (AIEE) and excited-state intramolecular proton transfer (ESIPT) phenomena.
研究成果
Probe 2 successfully combines AIEE and ESIPT phenomena for highly selective and sensitive detection of Al3+ ions with a low detection limit of 10 nM. The "turn-on" fluorescence is attributed to metal-induced aggregation, as confirmed by spectroscopic and microscopic analyses. This probe holds promise for applications in environmental monitoring and organic luminescent materials.
研究不足
The study is limited to in vitro conditions using pure solvents; potential interference from certain metal ions like Fe3+, Cu2+, and Pb2+ may affect detection accuracy; scalability and real-world application in environmental or biological samples require further validation.
1：Experimental Design and Method Selection:
The study involved designing a molecular probe (probe 2) based on tetraphenylethylene (TPE) and Schiff base units to exploit AIEE and ESIPT for sensing Al3+ ions. Methods included synthesis, photophysical characterization (UV-vis and fluorescence spectroscopy), and theoretical calculations (DFT and TD-DFT).
2：Sample Selection and Data Sources:
Probe 2 was synthesized from compound 1 and salicylaldehyde. Metal ions (e.g., Na+, K+, Al3+, etc.) in perchlorate salt forms were used for sensing studies in methanol solvent.
3：List of Experimental Equipment and Materials:
Equipment included NMR spectrometer (JEOL ECS-400 MHz), mass spectrometer (BRUKER DALTONIK microTOF-Q11), UV-vis spectrophotometer (Shimadzu-2600), fluorescence spectrophotometer (Varian Cary Eclipse), FE-SEM (ZEISS MERLIN Compact), TEM (FP 5022/22-Tecnai G2 20 S-TWIN), and computational software (Gaussian 09W). Materials included benzophenone, titanium tetrachloride, zinc powder, salicylaldehyde, solvents (THF, CH3OH, CH3CN), and metal perchlorates.
4：Experimental Procedures and Operational Workflow:
Synthesis of probe 2 via reflux reaction; characterization using NMR, MS; photophysical studies with varying solvent ratios and metal ion additions; FE-SEM/TEM for morphological analysis; DLS for size measurement; theoretical optimizations using DFT/B3LYP and TD-DFT.
5：Data Analysis Methods:
Binding constants calculated using Benesi-Hildebrand equation; detection limits determined from emission intensity vs. concentration plots; statistical analysis of blank measurements for signal-to-noise ratio.
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