1：Experimental Design and Method Selection:

The study involved the synthesis of three carbazole-based tetraphenylethene derivatives (compounds 1-3) using palladium-catalyzed coupling reactions. The AIE behaviors were investigated using photoluminescence spectroscopy in DMF-water mixtures, and mechanofluorochromic properties were studied through grinding and solvent vapor exposure, with powder X-ray diffraction (PXRD) to analyze morphological changes.

2：Sample Selection and Data Sources:

Compounds 1-3 were synthesized from intermediate products (1-1, 1-2, 1-3, and 1-4) as described in the literature. Solutions were prepared in DMF with varying water fractions for aggregation studies, and solid samples were used for fluorescence and mechanochromism tests.

3：List of Experimental Equipment and Materials:

Equipment included a Varian Mercury Plus 400 NMR spectrometer, Bruker AmaZon SL Ion Trap Mass spectrometer, PE CHN 2400 analyzer, Agilent 8453 UV/Vis spectrophotometer, Hitachi-F-4600 fluorescence spectrophotometer, Shimadzu XRD-6000 diffractometer, Edinburgh FLS1000 spectrometer for quantum yields and lifetimes, and NanoBrook 90Plus for dynamic light scattering. Materials included various boronic acids, palladium catalysts, solvents (e.g., DMF, toluene, dichloromethane), and silica gel for chromatography.

4：Experimental Procedures and Operational Workflow:

Synthesis involved refluxing intermediates with catalysts in toluene, followed by extraction and purification. For AIE studies, DMF solutions of compounds were mixed with water, and PL spectra were recorded. For mechanochromism, solid samples were ground with a pestle, treated with dichloromethane vapor, and analyzed using PXRD and PL spectroscopy.

5：Data Analysis Methods:

Data were analyzed using NMR for structural confirmation, mass spectrometry for molecular weight, UV/Vis and PL spectroscopy for optical properties, PXRD for crystallinity, and DLS for aggregate size distribution. Quantum yields and lifetimes were measured using the Edinburgh FLS1000 spectrometer.