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Lysosome-Targeted Single Fluorescence Probe for Two-Channel Imaging Intracellular SO2 and Biothiols
摘要： As the members of reactive sulfur species, SO2 and biothiols play a signi?cant role in physiological and pathological processes and directly in?uence numerous diseases. Furthermore, SO2 and biothiols can provide a reductive environment for lysosomes to carry out their optimal functionality. To this end, the development of single ?uorescent probes for imaging SO2 and biothiols from different emission channels is highly desirable for understanding their physiological nature. Here, a lysosome-targeted ?uorescent probe (BPO-DNSP) with a dual reaction site for SO2 and biothiols was presented. BPO-DNSP can sensitively and selectively respond to SO2 in the green channel with a large Stokes shift over 105 nm, and to biothiols in the near-infrared emission channel with a large Stokes shift over 109 nm. The emission shift for the two channels was as high as 170 nm. Colocalization experiments veri?ed that BPO-DNSP can selectively enrich lysosomes. Notably, BPO-DNSP can not only be used to image intracellular SO2 and biothiols from two different channels, but also to monitor the conversion of biothiols to SO2 without adding exogenous enzymes in living HeLa cells.
关键词： ?uorescence imaging,lysosome-targeted,single ?uorescent probe,biothiols,SO2
更新于2025-11-21 11:08:12
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A Colorimetric/Fluorescence Dual-channel Probe for Highly Discriminating Detection of Cysteine
摘要： In this work, a novel fluorescence (FL) probe for selective and sensitive detection of Cys with colorimetric and FL dual signal changes was reported. The probe was synthesized by two step of sulfonamide reaction coupling between a sulfonyl benzoxadiazole (SBD) dye and dansyl chloride linked with rigid piperazine group. The probe showed a specific off-on response to Cys in aqueous solution with nanomolar LOD, and without interference by a range of amino acids and several competing analytes. Upon addition of Cys, the probe will undergo sequential substitution and intramolecular rearrangement reactions, yielding a 4-amino SBD derivative, which results in generation of strong yellow fluorescence emission at 575 nm accompanied by a two-step red shift in the absorption spectral. Moreover, it can be used for imaging of endogenous Cys in living cells.
关键词： Fluorescence probe,Image analysis,Biothiols,Colorimetric
更新于2025-09-23 15:21:01
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L-cystine-linked BODIPY-adsorbed monolayer MoS2 quantum dots for ratiometric fluorescent sensing of biothiols based on the inner filter effect
摘要： This study fabricated a dual-emission probe consisting of monolayer MoS2 quantum dots (M-MoS2 QDs) and L-cystine-linked boron-dipyrromethene (L-Cys-BODIPY) molecules for ratiometric sensing of biothiols, thiol product-related enzyme reactions, and ratiometric imaging of glutathione (GSH)-related reactions in HeLa cells. The formation of L-Cys-BODIPY-adsorbed M-MoS2 QDs (named as BODIPY-M-MoS2 QDs) was demonstrated by comparing them with M-MoS2 QDs using transmission electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy. The BODIPY-M-MoS2 QDs exhibited dual-emission bands, excellent biocompatibility, and good resistance to photobleaching. It was found that the adsorbed L-Cys-BODIPY molecules rarely quenched the fluorescence of M-MoS2 QDs, and meanwhile, they were self-quenched by π-π stacking between each BODIPY backbones. The presence of biothiols induced the reduction of weakly fluorescent L-Cys-BODIPY to strongly fluorescent of L-cysteine-conjugated BODIPY. Since having a much higher molar absorption coefficient than L-Cys-BODIPY, the liberated L-cysteine-conjugated BODIPY behaved as an effective inner filter to absorb the excitation light and subsequently quenched the fluorescence of M-MoS2 QDs. The appearance of L-cysteine-conjugated BODIPY could barely affected to the fluorescence lifetime of M-MoS2 QDs, confirming the inner filter effect of L-cysteine-conjugated BODIPY onto the fluorescence of M-MoS2 QDs. The present probe not only provided a linear ratiometric response to 1–10 mM GSH, 1-10 μM cysteine, and 1-10 μM of homocysteine but also remarkably showed the ratiometric detection of thiol products from the reactions of 1–900 units L-1 S-adenosylhomocysteine (SAH) hydrolase and SAH as well as 1-850 units L-1 GSH reductase and disulfide GSH. Additionally, the present probe was well-suited for ratiometric imaging of intracellular GSH levels in non-treated and drug-treated HeLa cells
关键词： ratiometric imaging,BODIPY,biothiols,ratiometric sensor,MoS2 quantum dots,enzymatic reaction
更新于2025-09-23 15:21:01
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Black phosphorus quantum dots are useful oxidase mimics for colorimetric determination of biothiols
摘要： Black phosphorus quantum dots (BP QDs) with small size are synthesized using an easy to operate thermal method. It was found that BP QDs possess oxidase-mimicking activity. They can catalyze the oxidation of the substrate 3,3′,5,5′-tetramethylbenzidine to produce a blue-colored product even in the absence of hydrogen peroxide. Active oxygen species are proved to be involved in the reaction through the experiments of radical scavenging and electron spin resonance. Biothiols including reduced glutathione and cysteine inactivate the oxidase-mimicking activity of BP QDs, concomitant to the fading of the blue solution. This provides the base for a colorimetric method for the determination of glutathione and cysteine. The decreased absorbance at 652 nm displays linear response to the concentrations of glutathione ranging from 0.1 to 5.0 μmol L?1, and cysteine from 0.1 to 10.0 μmol L?1. The detection limits are 0.02 μmol L?1 and 0.03 μmol L?1 for glutathione and cysteine, respectively. Successive determinations of 1.0 μmol L?1 glutathione and 5.0 μmol L?1 cysteine solution give relative standard deviations of 0.8% and 1.7% (n = 11), respectively. As a preliminary application, the practicability of the method was evaluated by the determination of glutathione in pharmaceutical preparations. This work not only discovers a useful oxidase mimics but also sets up a reliable platform based on BP QDs in colorimetric detection.
关键词： Cysteine,Pharmaceutical,Nanoprobe,Reactive oxygen species,Black phosphorous quantum dots,Oxidase-mimicking activity,Glutathione,Biothiols,Colorimetric method
更新于2025-09-23 15:19:57
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A long-wavelength-emitting fluorescent probe for simultaneous discrimination of H2S/Cys/GSH and its bio-imaging applications
摘要： A long-wavelength fluorescent probe NR-CY was developed for simultaneous identification of cysteine / glutathione and sulphide by combining the derivative of Nile red with 7-nitrobenzofurazan. The response of NR-CY to thiols is regulated by intramolecular charge transfer and photoinduced electron transfer mechanisms. For sulphide at 560 nm, cysteine at 475 nm and glutathione at 425 nm, different absorbance increases can be observed. NR-CY can detect cysteine at fluorescence emission 543 nm and distinguish sulphide from other analytes by kinetic experiments at 636 nm. The probe showed a rapid response to these thiols (cysteine was 90 s and sulphide was 30 s). In addition, NR-CY has been successfully applied to live MCF-7 cell imaging.
关键词： biothiols,Fluorescent probe,cell imaging,long-wavelength-emitting
更新于2025-09-23 15:19:57
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Application of maleimide modified graphene quantum dots and porphyrin fluorescence resonance energy transfer in the design of a??a??turn-ona??a?? fluorescence sensors for biothiols
摘要： Two novel fluorescent probes were designed to detect the biothiol in foods using the highly efficient Michael addition reaction between maleimide-derived probes and the biothiol. First, maleimide functionalized GQDs (M-GQDs) were synthesized and used for biothiol identification according to the Michael addition principle. The biothiol can be detected in the range of 5× 10-9 to 4× 10-7 mol/L and the detection limit was 1.69×10-9 mol/L. Then, a fluorescence resonance energy transfer (FRET) system between M-GQDs and tetrakis (4-aminophenyl) porphyrin (TAPP) for biothiol detection was developed. However, the process of FRET was switched off in the presence of biothiols due to the switch of M-GQDs fluorescence emission to the“ON” mode following the Michael addition mechanism. The system could quickly and accurately detect the biothiol with a detection range of 6.7×10-10 to 2×10-7 mol/L and a detection limit of 2.34×10-10 mol/L. Compared to the single detection system, the FRET system had a wider detection range and lower detection limit, and the related biomolecules did not interfere with the quantitative identification of the biothiol. The proposed method was successfully applied for the determination of the biothiol in foods and human blood samples.
关键词： Porphyrin,Fluorescence “turn-on”,Biothiols,FRET,Graphene quantum dots
更新于2025-09-16 10:30:52
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Carbon quantum dot-AgOH colloid fluorescent probe for selective detection of biothiols based on the inner filter effect
摘要： Here, we present a selective and sensitive fluorescent probe for the detection and distinction of biothiols, such as glutathione (GSH) and cysteine (Cys). The adsorbance of Cys onto the surface of AgOH colloid could result in enhanced absorbance from 250 to 400 nm in the UV–vis absorption spectrum, while the addition of GSH could dissolve the AgOH colloid resulting in no change in the UV–vis absorption spectrum. Utilizing these different phenomena, two fluorescent probes were established based on the inner filter effect (IFE). The first probe, the “CDs-AgOH colloid” fluorescent probe, was used to quantitatively analyze Cys over a linear concentration range from 33 to 317 μM and a detection limit of 7.2 μM. The second probe, the “CDs-AgOH colloid-Cys” fluorescent probe, was used to quantitatively analyze GSH, with a detection limit down to 3.6 μM, and a linear range of detection of approximately 16.7 to 100 μM. The fluorescent probes were successfully applied for the detection of GSH in a fetal bovine serum (FBS) sample. Based on these results, IFE is considered to be an effective way to distinguish GSH and Cys.
关键词： Fluorescent probe,AgOH colloid,Carbon quantum dots,Inner filter effect,Biothiols
更新于2025-09-12 10:27:22
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A lysosome-targetable fluorescent probe for simultaneously sensing Cys/Hcy, GSH, and H2S from different signal patterns
摘要： Biothiols, a vital branch of reactive sulfur species (RSS) family, which are indispensable in human physiology. However, the exact functional roles of each biothiols involved in complicated physiological activities were still not fully clarified. A critical barrier is a lack of robust molecular tools which can simultaneously visualize different biothiols with distinct emission signals. Herein, the first lysosome-targetable fluorescent probe, Lyso-RC, which could respond to Cys/Hcy, GSH and H2S with different sets of signal patterns was developed. Lyso-RC responds to Cys/Hcy, GSH and H2S with the fluorescence signal patterns of blue-red, green-red, and red, respectively. Significantly, Lyso-RC is capable of discriminating lysosomal Cys/Hcy, GSH, and H2S in HeLa cells.
关键词： imaging,lysosome-targetable,simultaneous detection,fluorescent probe,biothiols
更新于2025-09-10 09:29:36
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Synthesis of Novel Organic-Inorganic Hybrid Fluorescent Microspheres and Their Applications as Fe(III), Hg(II) and Biothiols Probes
摘要： Organic-inorganic hybrid fluorescent microspheres (OI-FMs) without typical fluorophores were prepared. Fluorescence quantum yield of OI-FMs is up to 0.7 after being heated in an oven at 120°C (named as OIFMs-120). Interestingly, OI-FMs-120 shows different detection performances in different solvents. It can be used for qualitative and quantitative detection of Fe3+ with a detection limit of 37.8 nM in absolute ethanol. While in aqueous solution, it can be applied as an ONOFF-ON fluorescent probe. Fluorescence of the probe is quenched by Hg2+and later recovered by biothiols. The detection limit are as low as 34.9 nM for Hg2+, 91.0 nM for Cys, 0.12 μM for Hcy and 0.13 μM for GSH in aqueous medium, respectively.
关键词： Biothiols,Heavy metal ions,Atypical fluorophores,Fluorescence sensor
更新于2025-09-09 09:28:46