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[Methods in Molecular Biology] || Multifunctional Magnetic Nanoparticles-Labeled Mesenchymal Stem Cells for Hyperthermia and Bioimaging Applications
摘要： Magnetic nanoparticles have demonstrated considerable capacity for theranosis purposes due to their unique characteristics, including magnetic properties, comparable size to biomolecules, favorable conjugations of drugs and biomolecules, ability to labeling, and capability of sensing, separation, detection, and targeted drug delivery. They could be exploited in magnetic resonance imaging as the contrast agents and also warmed as exposed to an external magnetic AC ?eld that could be applied in hyperthermia. Here, progresses and advances in the strategy and assembly of ?uorescent magnetic nanoparticles are presented for stem cell tracing and drugs/biomolecules targeting into cells.
关键词： Conjugation,Fluorescent probe,Stem cell tracing,Magnetic resonance imaging,Magnetic nanoparticles,Hyperthermia
更新于2025-09-12 10:27:22
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Quantum dots based near-infrared fluorescent probe for the detection of PepT1 expression in colorectal cancer
摘要： The lysine-proline-valine tripeptide (KPV) can transport into colorectal cancer (CRC) cells via oligopeptide transporter 1 (PepT1) selectively. Here, we successfully designed a fluorescent probe for PepT1 receptor-targeted CRC imaging. This probe consisted of the CuInS2/ZnS (CIS/ZnS) quantum dots (QDs) conjugated with KPV (QD-PAAO-KPV), exhibiting excellent near-infrared emission characteristics. The in vitro and in vivo results showed that the QD-PAAO-KPV was specifically and efficiently internalized in the PepT1-overexpressing colorectal Caco-2 tumor. In addition, it demonstrated that the probe possessed good biological activities. Thus, this study revealed that the probe QD-PAAO-KPV presented desirable targeting ability, showing promising prospects in CRC detection.
关键词： Quantum dots,PepT1,Fluorescent probe,KPV Peptide,Tumor imaging
更新于2025-09-12 10:27:22
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Preparation and application of carbon quantum dots filled hollow mesoporous silica nanospheres
摘要： Hollow mesoporous silica and carbon quantum dots play an important role in the field of biological applications. In this study, a nanoscale hollow mesoporous silica nanosphere (HMSN) was used as the main body of a drug transport system, which filled with a small amount of carbon quantum dots. The hollow mesoporous silica structure provides a large specific surface area and cavity structure for efficient drug loading. And the carbon quantum dots could provide good fluorescence for imaging. By filling carbon quantum dots inside of silica nanospheres and in mesopores on the surface, the hollow mesoporous silica nanospheres became fluorescent and could be used as a fluorescent probe. And the potential photothermal effect of the internal carbon quantum dots also makes it possible to achieve controlled drug release through temperature changes.
关键词： fluorescent probe,carbon quantum dots,photothermal effect,HMSN
更新于2025-09-12 10:27:22
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Fluorometric determination of aflatoxin B1 using a labeled aptamer and gold nanoparticles modified with a complementary sequence acting as a quencher
摘要： A fluorometric aptamer based assay is described for rapid and sensitive detection of aflatoxin B1 (AFB1). It is making use of a fluorescein (FAM) labeled anti-AFB1 aptamer and complementary DNA-modified gold nanoparticles (GNPs). In the absence of AFB1, the FAM-labeled aptamers hybridize with complementary DNA strands that were covalently immobilized on GNPs. This results in quenching of the green fluorescence (with excitation/emission peaks at 485/525 nm). In the presence of AFB1, the aptamer probe binds AFB1 and is released from the GNPs. Hence, fluorescence is restored. Under optimized conditions, AFB1 in the concentration range from 61 pM to 4.0 μM can be detected, and the detection limit is 61 pM. This assay is highly selective for AFB1. It was applied to the determination of AFB1 spiked into 50-fold diluted wine and 20-fold diluted beer.
关键词： Mycotoxin,Food safety,Environmental analysis,Fluorescence quenching,Fluorophore,Fluorescent probe,Nanomaterials,Nanoprobe
更新于2025-09-11 14:15:04
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A Dicyanomethylene-4H-Pyran Based NIR Ratiometric Fluorescent Probe for Diazane and its Bioimaging
摘要： A near-infrared ICT-based fluorescent probe LX was successfully obtained. LX which detection limit is low as 22.2 nm shows excellent selectivity and high sensitivity to diazane. LX can selectively detected diazane from other species over a wide pH (3–10) range. A obvious color change of solution from yellow to orange can be found, allowing the naked eye to detect. The sensing mechanism was reasonably detected by ESI-MS and DFT calculations. In addition, LX succeed in the visualization of diazane in living cells and the detection of diazane in water samples.
关键词： Diazane,Fluorescent probe,ICT,NIR
更新于2025-09-10 09:29:36
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A near-infrared fluorescent probe based on SNAr reaction for H2S/GSH detection in living cells and zebrafish
摘要： Hydrogen sulfide (H2S) and biothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play critical roles in physiological and pathological processes. However, due to the challenge in sensing H2S and biothiols simultaneously, it is of great importance to solve this problem in biological processes. Herein, a near-infrared fluorescent probe (HZ-NBD) based on SNAr reactions was exploited to achieve the sensitive and selective detection of H2S and glutathione (GSH) simultaneously in the same conditions. The design strategy was employing dicyanoisophorone based fluorescent dye as the fluorophore, the NBD moiety as a response unit and a quencher of fluorophore. The probe exhibited a low limit of detection to H2S (13.2 nM) and GSH (112 nM) as well as a favorable large stokes shift (112 nm). The reaction mechanism was investigated via mass spectra. What’s more, owing to the advantages of low cytotoxicity, fast response and strong anti-interference ability, the probe HZ-NBD was successfully applied to bioimage H2S and GSH in HepG2 cells and zebrafish.
关键词： fluorescent probe,SNAr,dicyanoisophorone,near-infrared emission
更新于2025-09-10 09:29:36
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A ratiometric fluorescent probe for lysosomal hypochlorous acid based on through-bond energy transfer strategy
摘要： In this paper, we synthesized a ratiometric fluorescence probe (IRh-Ly) for lysosomal hypochlorous acid (HOCl) by adopting a through-bond energy transfer (TBET) strategy on rhodamine-imidazo[1,5-a]pyridine platform. IRh-Ly showed brilliant selectivity, rapid response for HOCl. The probe also exhibited high sensitivity with the detection limit calculated to be 10.2 nM. Moreover, we demonstrated its successful application of detecting lysosomal HOCl in living RAW264.7 cells. Notably, the morpholine was integrated into the fluorescent probe IRh-Ly and the results revealed that IRh-Ly could target lysosome and detect the lysosomal HOCl. All the unique features made IRh-Ly particularly suitable for ratiometric HOCl detection and bio-imaging applications.
关键词： Through-bond energy transfer,HOCl,Lysosomal,Ratiometric,Fluorescent probe
更新于2025-09-10 09:29:36
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A highly specific and ultrasensitive p-aminophenylether-based fluorescent probe for imaging native HOCl in live cells and zebrafish
摘要： It is very important to detect native hypochlorous acid (HOCl) in the complex biosystems owing to the important roles of HOCl in the immune defense and the pathogenesis of numerous diseases. In this paper, a new p-aminophenylether-based fluorescent probe PAPE-HA was developed for specific detection of HOCl. Probe PAPE-HA could implement the quantitative detection of HOCl ranging from 0 to 1 μM and the detection limit was obtained as low as 1.37 nM. Additionally, probe PAPE-HA could reach a rapid response for HOCl (< 2 min). Importantly, probe PAPE-HA with preeminent specificity and ultrasensitivity was proven to possess powerful capability of tracking native HOCl in live cells and zebrafish, and we thus anticipate that probe PAPE-HA could be used as a novel promising tool for revealing diverse cellular functions of HOCl.
关键词： hydroxynaphthalimide,hypochlorous acid (HOCl),Fluorescent probe,p-aminophenylether,bioimaging
更新于2025-09-10 09:29:36
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Phenanthrene-imidazole-based fluorescent sensor for selective detection of Ag+ and F? ions: real sample application and live cell imaging
摘要： 4-Methyl-2,6-bis(1H-phenanthro[9,10-d]imidazol-2-yl)phenol (probe-MPIP) conjugate has been designed as a selective fluorescent probe for silver (Ag+) ions. The probe-MPIP exhibits selectivity towards Ag+ in DMSO-H2O (1:9 v/v) in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) at pH 7.4. Moreover, the detection of probe-MPIP towards Ag+ ions could be realized with a “naked eye” with a color conversion from greenish yellow to orange. The linear relationship between fluorescence intensity and Ag+ ion concentration indicates that probe-MPIP can be used for quantification of Ag+ ions. The probe-MPIP can be used in a broad range of pH from 3 to 11. The limit of detection (LOD) and association constant (Ka) of MPIP-Ag+ complex have been determined and found to be 1.8 nM and 8.24 × 105 M?1, respectively, according to fluorescence titrations. Moreover, the binding ratio of probe-MPIP and Ag+ was found to be 1:1 by Job’s method. Furthermore, MPIP-Ag+ complex subsequently acted as a secondary fluorescent turn-on probe for fluoride (F?) ion recognition among the other anions. To assess the real-world efficacy of probe-MPIP and MPIP-Ag+ complex, a real water sample of silver and fluoride ions (Ag+ and F?) was evaluated. Furthermore, probe-MPIP and MPIP-Ag+ complex have been successfully applied to cell imaging studies.
关键词： Fluorescent probe,Silver and fluoride ion detection,Binding constant,In vitro cell imaging,Real sample analysis
更新于2025-09-10 09:29:36
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A lysosome-targetable fluorescent probe for simultaneously sensing Cys/Hcy, GSH, and H2S from different signal patterns
摘要： Biothiols, a vital branch of reactive sulfur species (RSS) family, which are indispensable in human physiology. However, the exact functional roles of each biothiols involved in complicated physiological activities were still not fully clarified. A critical barrier is a lack of robust molecular tools which can simultaneously visualize different biothiols with distinct emission signals. Herein, the first lysosome-targetable fluorescent probe, Lyso-RC, which could respond to Cys/Hcy, GSH and H2S with different sets of signal patterns was developed. Lyso-RC responds to Cys/Hcy, GSH and H2S with the fluorescence signal patterns of blue-red, green-red, and red, respectively. Significantly, Lyso-RC is capable of discriminating lysosomal Cys/Hcy, GSH, and H2S in HeLa cells.
关键词： imaging,lysosome-targetable,simultaneous detection,fluorescent probe,biothiols
更新于2025-09-10 09:29:36