研究目的
To synthesize and examine the impact of free Eudragit RS 100 nanoparticles (LN01), Quantum dots curcumin-loaded Eudragit RS 100 nanoparticles (LN04), and un-encapsulated curcumin nanoparticles (LN06) on cancerous and bacterial cells.
研究成果
Curcumin-loaded Eudragit nanoparticles (LN04) caused profound inhibition in colon and breast cancer cell growth and proliferation compared with nonencapsulated curcumin. LN04 selectively targeted the cancer cells and induced programmed cell death. Encapsulated curcumin in the Eudragit showed better inhibition of bacterial growth as compared with nonencapsulated curcumin.
研究不足
The study does not explore the molecular mechanism by which LN01, LN04, and LN06 induced apoptosis in the cancer cells. The antibacterial properties were only tested on one strain of E. coli.
1：Experimental Design and Method Selection:
Synthesis of LN01, LN04, and LN06 using solvent displacement technique. Characterization by FTIR, ζ potential, UV–Vis spectroscopy, TEM, and SEM. Biological activities evaluated by MTT assay, DAPI staining, and colony assay.
2：Sample Selection and Data Sources:
Human colorectal carcinoma cells (HCT-116), breast cancer cells (MCF-7), and normal cells (HEK-293) were used. Bacterial strain Escherichia coli was selected for antimicrobial activity.
3：List of Experimental Equipment and Materials:
Zetasizer Nano ZS200, IRAf?nity-1 FTIR spectrometer, UV–Vis spectrophotometer, SEM (FEI, Inspect S50), TEM (FEI, Morgagni 268), CO2 incubator (Heracell 150), confocal scanning microscope (Zeiss).
4：Experimental Procedures and Operational Workflow:
Cells were treated with different dosages of LN01, LN04, and LN06. After 48 h, cell viability was checked by MTT assay. Nuclear staining by DAPI was performed to examine the influence on cell nucleus. Colony formation ability was examined following crystal violet staining.
5：After 48 h, cell viability was checked by MTT assay. Nuclear staining by DAPI was performed to examine the influence on cell nucleus. Colony formation ability was examined following crystal violet staining. Data Analysis Methods:
5. Data Analysis Methods: Statistical analyses were completed with GraphPad Prism 6. The difference between the control and treated groups was calculated by a one-way analysis of variance.
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UV–Vis spectrophotometer
V-750
JASCO
To measure the percentage absorbance of the QDs to visible light.
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SEM
Inspect S50
FEI
To observe the wide-range surface morphology of QDs and curcumin in the dispersion.
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TEM
Morgagni 268
FEI
To evaluate the size, shape and structure of QDs and curcumin at high resolution.
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CO2 incubator
Heracell 150
Thermo Fisher Scientific
To incubate cells at 37?C in a CO2 environment.
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Zetasizer Nano ZS200
ZS200
Malvern Instruments
To calculate the mean size (Z-average), polydispersity index and ζ potential of nanoparticles.
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IRAf?nity-1 FTIR spectrometer
IRAf?nity-1
SHIMADZU
To obtain FTIR spectra of the prepared specimens.
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Confocal scanning microscope
Zeiss
To examine the staining of cells.
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