研究目的
Developing accurate and sensitive assays for H2Sn detection in biological science.
研究成果
HQO-PSP is a novel fluorescence probe that shows a significant fluorescence emission increase as a fast response to mitochondrial H2Sn. It is highly sensitive and selective towards H2Sn and can monitor changes of mitochondrial H2Sn in living cells and animal tissues, making it a useful tool to investigate the roles of mitochondrial H2Sn in physiopathology.
研究不足
The study focuses on the detection of H2Sn in living cells and tissues using a specific fluorescent probe, HQO-PSP. The limitations include the need for further validation in more complex biological systems and potential interference from other reactive species in vivo.
1：Experimental Design and Method Selection:
The study involved the synthesis of a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, for tracking H2Sn. The probe's reaction with H2Sn was explored, and its fluorescent properties were investigated.
2：Sample Selection and Data Sources:
A549 cells and mouse lung tissues were used as samples.
3：List of Experimental Equipment and Materials:
Spectrofluorophotometer (Shimadzu RF-6000), confocal microscope, and various chemicals including Na2S2 and Na2S
4：Experimental Procedures and Operational Workflow:
The probe's reaction with H2Sn was monitored over time, and its application in living cells and tissues was tested through fluorescence imaging.
5：Data Analysis Methods:
Fluorescence intensity was measured and analyzed to determine the probe's sensitivity and selectivity towards H2Sn.
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